Isolation of High-Molecular-Weight DNA from Monolayer Cultures of Mammalian Cells Using Proteinase K and Phenol.
نویسندگان
چکیده
This procedure is the method of choice for purification of mammalian genomic DNA from monolayer cultures when large amounts of DNA are required, for example, for Southern blotting. Approximately 200 µg of mammalian DNA, 100-150 kb in length, is obtained from 5 × 107 cultured aneuploid cells (e.g., HeLa cells).
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ورودعنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2017 7 شماره
صفحات -
تاریخ انتشار 2017